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Selection and Characterization of Potential Baker’s Yeast from Indigenous Resources of Nepal

Title: Selection and Characterization of Potential Baker’s Yeast from Indigenous Resources of Nepal
Authors: Karki, Tika B.; Timilsina, Parash Mani; Yadav, Archana; Pandey, Gyanu Raj; Joshi, Yogesh; Bhujel, Sahansila; Adhikari, Rojina; Neupane, Katyayanee
Contributors: Kyushu University
Source: Biotechnology Research International ; volume 2017, page 1-10 ; ISSN 2090-3138 2090-3146
Publisher Information: Wiley
Publication Year: 2017
Collection: Wiley Online Library (Open Access Articles via Crossref)
Description: The study aims to isolate the yeast strains that could be used effectively as baker’s yeast and compare them with the commercial baker’s yeast available in the market of Nepal. A total of 10 samples including locally available sources like fruits, Murcha, and a local tree “Dar” were collected from different localities of Bhaktapur, Kavre, and Syangja districts of Nepal, respectively. Following enrichment and fermentation of the samples, 26 yeast strains were isolated using selective medium Wallerstein Laboratory Nutrient Agar. From the differential tests which included morphological and microscopic observation and physiological and biochemical characterization such as nitrate reduction and lactose utilization tests, 8 strains were selected as possible Saccharomyces strain. The selected strains were further assessed for their efficient leavening ability by tests such as ethanol tolerance, osmotolerance, invertase test, and stress exclusion test. The three most potent strains ENG, MUR3B, and SUG1 isolated from grape, Murcha, and sugarcane, respectively, were used in the fermentation and baking of dough. These strains also carried a possibility of being used as industrial baker’s yeast.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1155/2017/1925820
Availability: https://doi.org/10.1155/2017/1925820; http://downloads.hindawi.com/archive/2017/1925820.pdf; http://downloads.hindawi.com/archive/2017/1925820.xml
Rights: http://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.FE678F34
Database: BASE