| Title: |
Reassignment of the murine 3'TRDD1 recombination signal sequence. |
| Authors: |
Touvrey, Cédric; Cowell, Lindsay, G.; Lieberman, Ann, E.; Marche, Patrice, N.; Jouvin-Marche, Evelyne; Candéias, Serge, M. |
| Contributors: |
Contrôle moléculaire de la réponse immune specifique; Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM); Departments of Biostatistics & Bioinformatics and Immunology; Duke University Durham; This work was supported in part by grant from “l'Association pour la Recherche contre le Cancer”, and from the Burroughs Wellcome Fund. |
| Source: |
ISSN: 0093-7711. |
| Publisher Information: |
CCSD; Springer Verlag |
| Publication Year: |
2006 |
| Collection: |
Université Grenoble Alpes: HAL |
| Subject Terms: |
T cell receptor gene; V(D)J recombination; recombination signal; signal joint; junctional diversity; [SDV.IMM]Life Sciences [q-bio]/Immunology |
| Description: |
T cell receptor genes are assembled in developing T lymphocytes from discrete V, D, and J genes by a site-specific somatic rearrangement mechanism. A flanking recombination signal, composed of a conserved heptamer and a semiconserved nonamer separated by 12 or 23 variable nucleotides, targets the activity of the rearrangement machinery to the adjoining V, D, and J genes. Following the rearrangement of V, D, or J genes, their respective recombination signals are ligated together. Although these signal joints are allegedly invariant, created by the head-to-head abuttal of the heptamers, some do exhibit junctional diversity. Recombination signals were initially identified by comparison and alignment of germ-line sequences with the sequence of rearranged genes. However, their overall low level of sequence conservation makes their characterization solely from sequence data difficult. Recently, computational analysis unraveled correlations between nucleotides at several positions scattered within the spacer and recombination activity, so that it is now possible to identify putative recombination signals and determine and predict their recombination efficiency. In this paper, we analyzed the variability introduced in signal joints generated after rearrangement of the TRDD1 and TRDD2 genes in murine thymocytes. The recurrent presence of identical nucleotides inserted in these signal joints led us to reconsider the location and sequence of the TRDD1 recombination signal. By combining molecular characterization and computational analysis, we show that the functional TRDD1 recombination signal is shifted inside the putative coding sequence of the TRDD1 gene and, consequently, that this gene is shorter than indicated in the databases. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/17021860; PUBMED: 17021860 |
| DOI: |
10.1007/s00251-006-0150-1 |
| Availability: |
https://inserm.hal.science/inserm-00089245; https://inserm.hal.science/inserm-00089245v1/document; https://inserm.hal.science/inserm-00089245v1/file/Touvrey-Immunogenetics-in_press.pdf; https://doi.org/10.1007/s00251-006-0150-1 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.FE731FE5 |
| Database: |
BASE |