| Title: |
Differences in the Transduction of Canonical Wnt Signals Demarcate Effector and Memory CD8 T Cells with Distinct Recall Proliferation Capacity |
| Authors: |
Boudousquié, Caroline; Danilo, Maxime; Pousse, Laurène; Jeevan-Raj, Beena; Angelov, Georgi S; Chennupati, Vijaykumar; Zehn, Dietmar; Held, Werner |
| Source: |
The Journal of Immunology ; volume 193, issue 6, page 2784-2791 ; ISSN 0022-1767 1550-6606 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2014 |
| Description: |
Protection against reinfection is mediated by Ag-specific memory CD8 T cells, which display stem cell–like function. Because canonical Wnt (Wingless/Int1) signals critically regulate renewal versus differentiation of adult stem cells, we evaluated Wnt signal transduction in CD8 T cells during an immune response to acute infection with lymphocytic choriomeningitis virus. Whereas naive CD8 T cells efficiently transduced Wnt signals, at the peak of the primary response to infection only a fraction of effector T cells retained signal transduction and the majority displayed strongly reduced Wnt activity. Reduced Wnt signaling was in part due to the downregulation of Tcf-1, one of the nuclear effectors of the pathway, and coincided with progress toward terminal differentiation. However, the correlation between low and high Wnt levels with short-lived and memory precursor effector cells, respectively, was incomplete. Adoptive transfer studies showed that low and high Wnt signaling did not influence cell survival but that Wnt high effectors yielded memory cells with enhanced proliferative potential and stronger protective capacity. Likewise, following adoptive transfer and rechallenge, memory cells with high Wnt levels displayed increased recall expansion, compared with memory cells with low Wnt signaling, which were preferentially effector-like memory cells, including tissue-resident memory cells. Thus, canonical Wnt signaling identifies CD8 T cells with enhanced proliferative potential in part independent of commonly used cell surface markers to discriminate effector and memory T cell subpopulations. Interventions that maintain Wnt signaling may thus improve the formation of functional CD8 T cell memory during vaccination. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.4049/jimmunol.1400465 |
| Availability: |
https://doi.org/10.4049/jimmunol.1400465; https://academic.oup.com/jimmunol/article-pdf/193/6/2784/61495585/1400465.pdf |
| Rights: |
https://academic.oup.com/pages/standard-publication-reuse-rights |
| Accession Number: |
edsbas.FEDD6B45 |
| Database: |
BASE |