| Title: |
www.mdpi.com/journal/ijms Swelling-Activated Anion Channels Are Essential for Volume Regulation of Mouse Thymocytes |
| Authors: |
Ranokhon S. Kurbannazarova; Svetlana V. Bessonova; Yasunobu Okada; Ravshan Z. Sabirov |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/02/2e/Int_J_Mol_Sci_2011_Dec_8_12(12)_9125-9137.tar.gz |
| Publication Year: |
2011 |
| Collection: |
CiteSeerX |
| Description: |
Channel-mediated trans-membrane chloride movement is a key process in the active cell volume regulation under osmotic stress in most cells. However, thymocytes were hypothesized to regulate their volume by activating a coupled K-Cl cotransport mechanism. Under the patch-clamp, we found that osmotic swelling activates two types of macroscopic anion conductance with different voltage-dependence and pharmacology. At the single-channel level, we identified two types of events: one corresponded to the maxi-anion channel, and the other one had characteristics of the volume-sensitive outwardly rectifying (VSOR) chloride channel of intermediate conductance. A VSOR inhibitor, phloretin, significantly suppressed both macroscopic VSOR-type conductance and single-channel activity of intermediate amplitude. The maxi-anion channel activity was largely suppressed by Gd 3+ ions but not by phloretin. Surprisingly, [(dihydroindenyl)oxy] alkanoic acid (DIOA), a known antagonist of K-Cl cotransporter, was found to significantly suppress the activity of the VSOR-type single-channel events with no effect on the maxi-anion channels at 10 μM. The regulatory volume decrease (RVD) phase of cellular response to hypotonicity was mildly suppressed by Gd 3+ ions and was completely |
| Document Type: |
text |
| File Description: |
application/zip |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.1301 |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.288.1301 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.FF20CA3B |
| Database: |
BASE |