| Title: |
Chronic dietary exposure to a glyphosate-based herbicide results in total or partial reversibility of plasma oxidative stress, cecal microbiota abundance and short-chain fatty acid composition in broiler hens |
| Authors: |
Mathias Fréville; Anthony Estienne; Christelle Ramé; Gaëlle Lefort; Marine Chahnamian; Christophe Staub; Eric Venturi; Julie Lemarchand; Elise Maximin; Alice Hondelatte; Olivier Zemb; Cécile Canlet; Rodrigo Guabiraba; Pascal Froment; Joëlle Dupont |
| Source: |
Frontiers in Physiology, Vol 13 (2022) |
| Publisher Information: |
Frontiers Media S.A., 2022. |
| Publication Year: |
2022 |
| Collection: |
LCC:Physiology |
| Subject Terms: |
birds; glyphosate; metabolism; cecal microbiome; oxidative stress; Physiology; QP1-981 |
| Description: |
Glyphosate-based herbicides (GBHs) are massively used in agriculture. However, few studies have investigated the effects of glyphosate-based herbicides on avian species although they are largely exposed via their food. Here, we investigated the potential reversibility of the effects of chronic dietary exposure to glyphosate-based herbicides in broiler hens. For 42 days, we exposed 32-week-old hens to glyphosate-based herbicides via their food (47 mg/kg/day glyphosate equivalent, glyphosate-based herbicides, n = 75) corresponding to half glyphosate’s no-observed-adverse-effect-level in birds. We compared their performance to that of 75 control animals (CT). Both groups (glyphosate-based herbicides and control animals) were then fed for 28 additional days without glyphosate-based herbicides exposure (Ex-glyphosate-based herbicides and Ex-control animals). Glyphosate-based herbicides temporarily increased the plasma glyphosate and AMPA (aminomethylphosphonic acid) concentrations. Glyphosate and aminomethylphosphonic acid mostly accumulated in the liver and to a lesser extent in the leg muscle and abdominal adipose tissue. Glyphosate-based herbicides also temporarily increased the gizzard weight and plasma oxidative stress monitored by TBARS (thiobarbituric acid reactive substances). Glyphosate-based herbicides temporarily decreased the cecal concentrations of propionate, isobutyrate and propionate but acetate and valerate were durably reduced. The cecal microbiome was also durably affected since glyphosate-based herbicides inhibited Barnesiella and favored Alloprevotella. Body weight, fattening, food intake and feeding behavior as well as plasma lipid and uric acid were unaffected by glyphosate-based herbicides. Taken together, our results show possible disturbances of the cecal microbiota associated with plasma oxidative stress and accumulation of glyphosate in metabolic tissues in response to dietary glyphosate-based herbicides exposure in broiler hens. Luckily, glyphosate-based herbicides at this concentration does not hamper growth and most of the effects on the phenotypes are reversible. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
1664-042X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fphys.2022.974688/full; https://doaj.org/toc/1664-042X |
| DOI: |
10.3389/fphys.2022.974688 |
| Access URL: |
https://doaj.org/article/a024be3601bb4aec99da23d0f504d2ec |
| Accession Number: |
edsdoj.024be3601bb4aec99da23d0f504d2ec |
| Database: |
Directory of Open Access Journals |