| Title: |
Timely bespoke phage-antibiotic combination to treat refractory Pseudomonas aeruginosa mediastinitis and vascular graft infection |
| Authors: |
Shimin Jasmine Chung; Yang Liu; Shuhua Thong; Yang Zhong; Zhi Soon Chong; Zhining Lim; Sabrina Tan; Jia Hao Yeo; Ming Guang Koh; Nathalie Grace Sy Chua; Dorothy Hui Lin Ng; Winnie Hui-Ling Lee; Tze Peng Lim; Limin Wijaya; Boon Huan Tan; Peng Huat Eric Yap; Thet Tun Aung; Rick Twee-Hee Ong; Karrie Kwan Ki Ko; Tse Hua Nicholas Wong; Yu Lin Charlene Tang; Yee Jim Loh; Teing Ee Tan; Thuan Tong Tan; Sandra Kolundzija; Wilfried Moreira; Andrea Lay-Hoon Kwa |
| Source: |
Nature Communications, Vol 17, Iss 1, Pp 1-11 (2026) |
| Publisher Information: |
Nature Portfolio, 2026. |
| Publication Year: |
2026 |
| Collection: |
LCC:Science |
| Subject Terms: |
Science |
| Description: |
Abstract Biofilm-related vascular graft infections (VGIs) pose major therapeutic challenges due to persistent, antibiotic-resistant bacteria often residing in retained grafts. Phage therapy offers a promising alternative treatment strategy against biofilm-associated infections, though its use remains mostly ad hoc and typically considered a last-resort intervention. We report here the treatment of a refractory, fluoroquinolone non-susceptible Pseudomonas aeruginosa VGI using a systematically planned and synergistic phage-antibiotic combination approach. Adjunctive phage therapy led to radiological improvement, as seen by reduced 18F-FDG PET/CT tracer uptake around the graft. The patient was transitioned to oral fluoroquinolone suppression therapy with no recurrence of bacteremia to-date, after a year. Our workflow led to the selection of phages that sensitized Pseudomonas aeruginosa to killing by levofloxacin and piperacillin-tazobactam. We established that this phage-driven antibiotic sensitization was due to the ability of our phages to use the MexAB-OprM efflux pump as a receptor. We also showed that our phages had potent anti-biofilm activity. We advocate a systematic, multi-pronged management strategy for refractory VGIs, including early therapeutic drug monitoring (TDM), in vitro antibiotic combination testing (iACT), and timely adjunctive phage therapy. This case illustrates the utility of individualized, strategic approaches and highlights adjunctive phage therapy’s potential in treating complex biofilm-related infections. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2041-1723 |
| Relation: |
https://doaj.org/toc/2041-1723 |
| DOI: |
10.1038/s41467-025-68136-y |
| Access URL: |
https://doaj.org/article/38f2e391a3a54960b4d00a9e44247dfc |
| Accession Number: |
edsdoj.38f2e391a3a54960b4d00a9e44247dfc |
| Database: |
Directory of Open Access Journals |