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MET-Driven Resistance to Sotorasib in KRAS G12C–Mutant NSCLC and Response to Combined KRAS and MET Inhibition

Title: MET-Driven Resistance to Sotorasib in KRAS G12C–Mutant NSCLC and Response to Combined KRAS and MET Inhibition
Authors: Richard Riedel, MD; Lea Ruge, MD; Malte Verheyen, MD; Felix John, MD; Heather Scharpenseel, MD; Lucia Nogova, MD, PhD; Sebastian Michels, MD; Rieke N. Fischer, MD; Anna Eisert, MD; Carolin Jakob, MD; Emanuel Niesen, MD; Jana Fassunke, MD; Janna Siemanowski-Hrach, MD; Carina Heydt, MD; Anne Bunck, MD; Udo Siebolts, MD, PhD; Sabine Merkelbach-Bruse, MD, PhD; Reinhard Buettner, MD, PhD; Jürgen Wolf, MD, PhD; Matthias Scheffler, MD, PhD
Source: JTO Clinical and Research Reports, Vol 7, Iss 3, Pp 100925- (2026)
Publisher Information: Elsevier, 2026.
Publication Year: 2026
Collection: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Subject Terms: NSCLC; KRAS G12C; Sotorasib; MET amplification; Tyrosine kinase inhibitor resistance; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; RC254-282
Description: Introduction: KRAS G12C mutations define a molecularly distinct subset of NSCLC for which targeted therapy with sotorasib has exhibited clinical efficacy. However, acquired resistance is inevitable. MET amplification has been described as a putative off-target resistance mechanism, although its clinical relevance remains incompletely understood. Methods: We conducted a retrospective case series of patients with KRAS G12C–mutant NSCLC treated with sotorasib at the University Hospital Cologne, Germany. Patients with available paired pre- and posttreatment biopsies were analyzed for resistance mechanisms using routine molecular diagnostics, including MET fluorescence in situ hybridization. Results: Nine patients with paired pre and posttreatment biopsies were identified. High-level MET amplification was detected by fluorescence in situ hybridization in four cases and intermediate-level amplification in one case after progression on sotorasib. Notably, one patient with acquired MET amplification achieved a renewed partial response to the combination of sotorasib and tepotinib after progression on sotorasib monotherapy. Conclusion: This study provides real-world evidence that MET amplification is an acquired and potentially targetable resistance mechanism to KRAS G12C inhibition in NSCLC. Our findings support rebiopsy at progression on sotorasib. Further prospective trials are warranted to validate MET amplification as a resistance mechanism and to define optimal therapeutic thresholds for combined KRAS and MET inhibition.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2666-3643
Relation: http://www.sciencedirect.com/science/article/pii/S2666364325001432; https://doaj.org/toc/2666-3643
DOI: 10.1016/j.jtocrr.2025.100925
Access URL: https://doaj.org/article/d4afc7c2d1fd45eea7dcf5b006bfeb67
Accession Number: edsdoj.4afc7c2d1fd45eea7dcf5b006bfeb67
Database: Directory of Open Access Journals