| Title: |
A novel detection method for lipid stability in almond leads to the discovery of marker-trait associations |
| Authors: |
Gina M. Sideli; Selina C. Wang; Brian J. Allen; Filipa S. Grilo; Thomas M. Gradziel |
| Source: |
Fruit Research, Vol 5, Iss 1, Pp 1-9 (2025) |
| Publisher Information: |
Maximum Academic Press, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Plant culture |
| Subject Terms: |
almond; lipid oxidation; shelf-life; kasp markers; Plant culture; SB1-1110 |
| Description: |
Nut kernel rancidity is a significant postharvest concern because it compromises flavor and decreases shelf-life. Roasting of nut kernels changes the chemical structure and leaves the kernel more susceptible to rancidity. This is highly dependent upon the composition of the nut kernel. Almonds contain fatty acids and phenolic compounds which can reduce the effects of rancidity. Understanding the genetic basis of rancidity susceptibility can assist breeding efforts for superior almond varieties. In this study, a rancimat machine was used to measure the rancidity time in a panel of 270 almond trees over two harvest years. This panel represents seven almond families, common cultivars, and peach accessions. The rancimat uses a heat block and controlled air flow to rapidly accelerate the oxidation process from weeks or months to hours, which makes it feasible to measure rancidity. Marker-trait associations (MTAs) were made for both single-year and multi-year analysis using data generated from the rancimat and genotyping-by-sequence data. Stable MTAs were discovered on chromosome 2 for different models and years. Additional MTAs were found on chromosomes 1 and 8 which were in close proximity between years. Twenty-one almond trees, a subset of the original panel, that exhibited extended shelf-life from rancimat or exhibited a faster time to become rancid were further evaluation by biochemical analyses to confirm chemical composition. Three KASP marker assays were designed from these marker-trait associations and validated with a panel 52 almond genotypes for use in marker-assisted breeding. The markers developed from associated SNP on chromosomes 1 and 8 were found to be predictive of phenotype for shelf-life. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2769-4615 |
| Relation: |
https://doaj.org/toc/2769-4615 |
| DOI: |
10.48130/frures-0024-0012 |
| Access URL: |
https://doaj.org/article/4e92bc6ac37240cb8f4710b95fc2bfca |
| Accession Number: |
edsdoj.4e92bc6ac37240cb8f4710b95fc2bfca |
| Database: |
Directory of Open Access Journals |