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Platelet integrin αIIbβ3 plays a key role in a venous thrombogenesis mouse model

Title: Platelet integrin αIIbβ3 plays a key role in a venous thrombogenesis mouse model
Authors: Brian D. Adair; Conroy O. Field; José L. Alonso; Jian-Ping Xiong; Shi-Xian Deng; Hyun Sook Ahn; Eivgeni Mashin; Clary B. Clish; Johannes van Agthoven; Mark Yeager; Youzhong Guo; David A. Tess; Donald W. Landry; Mortimer Poncz; M. Amin Arnaout
Source: Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Publisher Information: Nature Portfolio, 2024.
Publication Year: 2024
Collection: LCC:Science
Subject Terms: Science
Description: Abstract Venous thrombosis (VT) is a common vascular disease associated with reduced survival and a high recurrence rate. VT is initiated by the accumulation of platelets and neutrophils at sites of endothelial cell activation. A role for platelet αIIbβ3 in VT is not established, a task complicated by the increased bleeding risk caused by partial agonists such as tirofiban. Here, we show that m-tirofiban, a modified version of tirofiban, does not agonize αIIbβ3 based on lack of neoepitope expression and the cryo-EM structure of m-tirofiban/full-length αIIbβ3 complex. m-tirofiban abolishes agonist-induced platelet aggregation while preserving clot retraction ex vivo and, unlike tirofiban, it suppresses venous thrombogenesis in a mouse model without increasing bleeding. These findings establish a key role for αIIbβ3 in VT initiation and suggest that m-tirofiban and compounds with a similar structurally-defined mechanism of action merit consideration as potential thromboprophylaxis agents in patients at high risk for VT and hemorrhage.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2041-1723
Relation: https://doaj.org/toc/2041-1723
DOI: 10.1038/s41467-024-52869-3
Access URL: https://doaj.org/article/5cb4e38292ca4761ba710d35e201c213
Accession Number: edsdoj.5cb4e38292ca4761ba710d35e201c213
Database: Directory of Open Access Journals