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Case Report: Molecular diagnostics and clinical courses of two adult spinal pilocytic astrocytoma long-term survivors with GTF2I::BRAF fusion

Title: Case Report: Molecular diagnostics and clinical courses of two adult spinal pilocytic astrocytoma long-term survivors with GTF2I::BRAF fusion
Authors: Lorenzo Argao; Pinar E. Zerk; Hsiang-Chih Lu; Zied Abdullaev; Martha Quezado; Michelle L. Cassidy; Bennett Mclver; Anna Choi; Marissa Panzer; Renee Tweneboah-Koduah; Lily Polskin; Marta Penas-Prado; Paul Park; Nathan Clarke; Kenneth Aldape; Jacob Mandel; Byram H. Ozer
Source: Frontiers in Oncology, Vol 16 (2026)
Publisher Information: Frontiers Media S.A., 2026.
Publication Year: 2026
Collection: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Subject Terms: BRAF fusion; case report; methylation profiling; pilocytic astrocytoma; spinal tumor; Neoplasms. Tumors. Oncology. Including cancer and carcinogens; RC254-282
Description: IntroductionPilocytic astrocytomas are driven by BRAF and mitogen-activated protein kinase (MAPK) alterations, typically KIAA1549::BRAF fusions. A rare GTF2I::BRAF fusion has been described, but little is known about these cases.Case reportHere, we report two cases with GTF2I::BRAF fusions. Case 1 is a 36-year-old man initially diagnosed with myxopapillary ependymoma at the conus medullaris with three recurrences over 23 years requiring two surgeries, three rounds of radiation therapy, and one round of lapatinib/temozolomide. A distant disease focus in T3/T4 was sampled and tested with modern diagnostic techniques revealing a pilocytic astrocytoma on histology and methylation profiling. The patient has subsequently had stable clinical and radiographic findings. Case 2 is another 36-year-old man initially diagnosed with meningitis and later neurosarcoid who underwent biopsy after 12 years when his spinal leptomeningeal disease continued to progress and an intraventricular non-enhancing nodule emerged as a separate focus. Sampling of the leptomeningeal disease led to a diagnosis of pilocytic astrocytoma by histology and a divergent methylation profile. The patient has remained neurologically stable under radiographic surveillance without any intervention.ResultsRadiographic, histological, and molecular data are presented for both cases and compared against the only other reported GTF2I::BRAF CNS case, as well as canonical versions of pilocytic astrocytoma.ConclusionTo our knowledge, this is only the second case series highlighting a unique GTF2I::BRAF fusion and the first to describe it in adults in a spinal location. The manuscript contributes documentation of a rare fusion and tumor presentation to guide clinicians and potential research avenues.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2234-943X
Relation: https://www.frontiersin.org/articles/10.3389/fonc.2026.1670639/full; https://doaj.org/toc/2234-943X
DOI: 10.3389/fonc.2026.1670639
Access URL: https://doaj.org/article/ac6f839ddfbf4a6ca0b209bc14f59cd7
Accession Number: edsdoj.6f839ddfbf4a6ca0b209bc14f59cd7
Database: Directory of Open Access Journals