| Title: |
Antioxidant Bioactive Peptides from By-Product Canned Yellowfin Tuna (Thunnus sp.) through an Enzymatic Membrane Reactor (EMR) |
| Authors: |
Hardoko Hardoko; Azahraa K. Priantono; Flavia D. Reda; Tunggul S. Haji; Azis B. Sitanggang; Yunita E. Puspitasari |
| Source: |
Squalen, Vol 20, Iss 2 (2025) |
| Publisher Information: |
Kementerian Kelautan dan Perikanan, 2025. |
| Publication Year: |
2025 |
| Collection: |
LCC:Ocean engineering; LCC:Naval Science |
| Subject Terms: |
body juice; pre-cooking; amino acid; free radicals; oxidation; Ocean engineering; TC1501-1800; Naval Science |
| Description: |
As a by-product of the pre-cooking phase, canned yellow fin tuna (Thunnus sp.) is processed into body juice. A bioactive peptide derived from an organism#39;s body juice was developed to create a functional food. Hence, the purpose of the research was to evaluate the biological activity of antioxidant bioactive peptides prepared from body juice. Three stages were conducted to determine the substrate-to-enzyme and enzyme-to-substrate ratios for the production of antioxidative bioactive peptides in a batch reactor. These stages include the production of antioxidative bioactive peptides in an automated EMR with a constant flux and residence time, as well as the production of bioactive peptides using ultrafiltration with membranes of 10, 5, 4, and 2 kDa. The process was conducted at a temperature of 50°C, pH level of 7, constant flux of 8.08 L/m 2 /h, enzyme concentration of 15%, and substrate concentration of 2%. Furthermore, the optimal treatment implemented an automated EMR and filtered through a membrane of size 4 kDa, generating an IC 50 value of 0.604 mg/mL for antioxidant activity. The LC–MS analysis results indicated that the bioactive peptide, which passed through a 4 kDa membrane, contained the amino acid phenylalanine. Therefore, it can be inferred that an automated EMR could potentially be applied to create bioactive peptides from the body juice of by-products of canned yellow fin tuna (Thunnus sp.) that exhibit antioxidant activity. |
| Document Type: |
article |
| File Description: |
electronic resource |
| Language: |
English |
| ISSN: |
2089-5690; 2406-9272 |
| Relation: |
https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/980; https://doaj.org/toc/2089-5690; https://doaj.org/toc/2406-9272 |
| DOI: |
10.15578/squalen.980 |
| Access URL: |
https://doaj.org/article/f6bb79d3fbe04c8d8ecf538c75bbeb75 |
| Accession Number: |
edsdoj.f6bb79d3fbe04c8d8ecf538c75bbeb75 |
| Database: |
Directory of Open Access Journals |