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Automated quantification of fine root production from minirhizotron image time series

Title: Automated quantification of fine root production from minirhizotron image time series
Authors: Alexander Gillert; Patrick Möhl; Bo Peters; Lionel Safar; Julia Bebout; Stuart Schwab; Erika Hiltbrunner; Jürgen Kreyling; Tara Javidi; Elsa Cleland
Source: Methods in Ecology and Evolution, Vol 17, Iss 3, Pp 758-767 (2026)
Publisher Information: Wiley, 2026.
Publication Year: 2026
Collection: LCC:Ecology; LCC:Evolution
Subject Terms: biogeochemical cycles; computer vision; machine learning; minirhizotrons; root production; Ecology; QH540-549.5; Evolution; QH359-425
Description: Abstract Plant root growth accounts for a major part of the net primary production in grassland and forest ecosystems and influences the global carbon and nutrient cycles. Measuring the production of roots is inherently difficult, prone to inconsistencies and time‐consuming. Notably, there are currently no methods yet to automate this task. We have developed GINGER, a new method for automated estimation of the fine root production from a time series of minirhizotron images. It compares pairs of consecutive images with each other, separating new root growth from standing crop. The method was evaluated on four datasets from grassland, drained fen peatland and forest ecosystems. It exhibits performance on a similar level to that of human annotators while substantially reducing the time required for the data analysis. Human annotators showed a significant degree of variability among each other, confirming that the task is subjective and error‐prone. For demonstration, this pipeline was applied on two real‐world image datasets, spanning 2 and 3 years, to compute the total annual root production. End‐to‐end, including annotation and model training, GINGER reduced the required human workload from several thousand to less than 40 work hours. It could allow to scale up monitoring efforts and enable full automation in the future.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2041-210X
Relation: https://doaj.org/toc/2041-210X
DOI: 10.1111/2041-210x.70257
Access URL: https://doaj.org/article/cff82fdf2e874ba39fdf1626d4f7fbf2
Accession Number: edsdoj.ff82fdf2e874ba39fdf1626d4f7fbf2
Database: Directory of Open Access Journals