| Title: |
Fertility preservation in cancer patients : from cryopreserved ovarian tissue to isolated follicles |
| Authors: |
UCL - SSS/IREC/GYNE - Pôle de Gynécologie; UCL - Faculté de médecine et médecine dentaire; Dolmans, Marie-Madeleine; Andrade Amorim, Christiani; Tombal, Bertrand; Marbaix, Etienne; des Rieux, Anne; Leclercq, Isabelle; Smitz, Johan; Piver, Pascal; Luyckx, Valérie |
| Publisher Information: |
2014 |
| Document Type: |
Electronic Resource |
| Abstract: |
Ovarian tissue cryopreservation (OTC) and transplantation is a well established procedure to preserve fertility in young cancer patients. Although more than 30 live births have been achieved in adult patients so far, there are some concerns that need to be addressed when contemplating this approach: (i) Do ovarian follicles in cryopreserved ovarian tissue from prepubertal patients have the capacity to survive and develop properly after transplantation and exogenous stimulation? (ii) Is there a risk of reintroducing malignant cells after autotransplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer? (iii) Would it be possible to offer an alternative option to patients who cannot undergo transplantation of frozen-thawed ovarian tissue? The aim of this thesis was to answer these specific questions. For this reason, it was divided into three parts: Transplantation of cryopreserved ovarian tissue from prepubertal patients After 21 weeks of transplantation to immunodeficient mice and exogenous hormonal stimulation, prepubertal ovarian follicles were able to survive and grow up to the antral stage, proving their sensitivity to gonadotropins. Follicular density was high in prepubertal ovarian tissue and remained so after transplantation, with a preserved primordial follicle pool, suggesting a longer lifespan of grafted ovarian tissue. Safety of ovarian tissue transplantation: the specific issue of breast cancer patients While transplantation of frozen-thawed ovarian tissue from early-stage breast cancer patients is reportedly to be safe, our study with advanced-stage breast cancer patients confirms that there is a potential risk of reimplanting metastatic cells in these subjects, as some cells expressing the MGB2 gene were detected after grafting. However, the real malignant potential of these cells is not known, as transplantation of frozen-thawed ovarian tissue from advanced-stage breast cancer patients to immunodeficient mice did not; (MED - Sciences médicales) -- UCL, 2014 |
| Index Terms: |
info:eu-repo/semantics/doctoralThesis |
| URL: |
http://hdl.handle.net/2078.1/150531 |
| Availability: |
Open access content. Open access content; info:eu-repo/semantics/restrictedAccess |
| Note: |
English |
| Other Numbers: |
UCDLC oai:dial.uclouvain.be:boreal:150531; boreal:150531; 1130486991 |
| Contributing Source: |
UNIVERSITE CATHOLIQUE DE LOUVAIN; From OAIster®, provided by the OCLC Cooperative. |
| Accession Number: |
edsoai.on1130486991 |
| Database: |
OAIster |